THE EFFECTS OF Na-ASCORBATE ON ASCORBATE CONCENTRATION IN THE PLASMA AND TISSUES
Voja Pavlovic *
and Zoran Pavlovic **
Male guinea pigs of a
Hartley-derived strein, a mean starting weight of 325 g + 70 g (s.d.), immunized
with bovine serum albumine, were studied in animals maintained on various amount
of ascorbic acid for 28 days. Animals were paid-fed on ascorbate-free diet
(standard ''dry'' meal (Wagner Guinea Pig Diet). The animals were immunized with
bovine serum albumuine (BSA, Miles) 0.2 mg being administered in complete
Freund's adjuvans on day 0 (0.025 mg /foot pad and 0.1 mg in the nuchal skin)
and again on day 14 in 1% saline into the nuchal skin. The animals were
separated into five categories of five animals each and put on a daily schedule
of intraperitoneal injections of 0, 10, 25, 100 i 250 mg Na-ascorbate (Bronson).
Blood was taken by cardiac puncture from each animal on experimental days 0, 14
and 28. At the end of experiment all animals were anesthetized and tissue
samples were taken for evaluation of vitamin C with Zannoni methods. The
immunized guinea pigs receiving no supplementation showed a reduction in
ascorbic acid concentration of 59% at 14 days to 67% at 28 days. In the
meantime, no difference between the 10 and 25 mg doses in relative change.
Guinea pigs receiving the mega doses (100 mg and 250 mg/day) exhibited increased
plasma levels, the former showing a greater increase than latter. At the end of
the experiment the gain in the 100 mg group had dropped to 12%, but in the 250
mg group it continued to raise.