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Acta Medica Medianae
Vol. 46, No 3, Oktobar, 2005
UDK 61
YU ISSN 0365-4478





Gordana Bjelaković
Institut za biohemiju Medicinskog fakulteta
Bulevar dr Zorana Đinđića 81
18000 Niš, Srbija i Crna Gora

treatment of pain in aCUte abdomen

Gordana Bjelaković1, Tatjana Jevtović1 , Gordana Kostić2, Ivana Stojanović1 i Dušan Sokolović1

Institut za biohemiju Medicinskog fakulteta u Nišu1
Dečja interna klinika Kliničkog centra u Nišu2


The osmotic fragility test of erythrocytes is useful in the diagnosis of different types of hereditary hemolytic anemias followed with hyperbilirubinemia. Hemolytic anemias, characterized by accelerated destruction of red blood cells, are usually the consequence of many metabolic abnormalities like cellular membrane defect, erythrocyte enzymes defect or hemoglobin abnormalities – hemoglobinopathies. The object of our study was to assess the relationship between osmotic fragility test of erythrocytes and severity of indirect hyperbilirubinemia in some inherited erythrocytes’ disorders. We did the osmotic fragility test of erythrocytes by using Dacie, s method with normal values of erythrocytes hemolysis between 0,48 to 0,34% NaCl (minimal to maximal hemolysis). In hereditary spherocytosis, fragility of erythrocytes was increased (min. at 0,50 % NaCl to max. 0,44 % NaCl) . In the child with β- thalassemia and cycle cell anemia erythrocytes fragility was decreased (min . at 0,42 to max. 0,32 % NaCl), that is 0,40% min. of hemolysis and 0,34% max. hemolysis in the second case. In newborn infants with high levels of indirect bilirubin in serum as a cause of physiological jaundice, the osmotic fragility test was within a normal range. Our findings point out the diagnostic value of osmotic fragility test in assessing patients with the indirect hyperbilirubinemia. This simple and important diagnostic test can be performed in small laboratories. Acta Medica Medianae 2005; 44 (3): 47 – 51.


Key words: osmotic fragility test, erythrocytes, hyperbilirubinemia, childhood