Acta Medica Medianae
Number 1, Januar, 2006
YU ISSN 0365-4478
50dr Zorana Djindjic Street
18000 Nis, Serbia and Montenegro
Copyright 2006 by Faculty
of Medicine, University of Nis
IMPORTANCE OF HEMOCULTURE IN DIAGNOSIS OF
BACTEREMIA AND SEPSIS
Branislava Kocic, Dobrila Djordjević–Stankovic, Gordana Randjelovic
and Marina Dinic
Institute of Nis
Almost all kinds of microorganisms from blood can be isolated by
hemoculture. The isolate consists of 75–85% Gram negative and Gram
positive bacteria, until the other part of the isolate consists of fungi
or other microorganisms.
During the three-year period (1998–2001) we analyzed microbiological
characteristics of all positive hemocultures inspected in
microbiological laboratory in the Public Health Institute in Nis as well
as clinical parameters of patients with the aim of determining the cause
We applied standard microbiologic methods for inspection of 1995
hemocultures taken from 759 patients whereupon we registered positive
medical findings in 18,75%. In the majority of cases only one kind of
microorganisms was isolated, while in 1.06% of positive hemocultures we
isoleted two kinds of microorganisms.
heamolyticus gr. A, Streptococcus pneumoniae, E.coli, Proteus
mirabilisa, Salmonellae enteritidis, Pseudomonas aeruginose i Candide
Difteroids and Bacillus spp.
were isolated only from contaminated hemocultures.
is the most frequent contminant and causes bacteremia in the patients
with intravascular catheter and patients on intravenous and peritoneal
dialysis. A similar finding of α hemolitic
streptococcus of viridans group
was registered in the patients with subacute and acute endocarditis who
had had an intravenous catheter implanted.
On the basis of the results obtained, we can conclude that
microorganisms from groups of
Staphylococcus, Candida and Enterobacteriaceace
are the most frequent causes of bacteremia. Beside its limiting factors
(time of sampling, duration of incubation, possible contamination of
samples, number of hemocultures), hemoculture is the only method by
which the causes of bacteremia and sepsis can be isolated. Acta
Medica Medianae 2006;45(1):37-42.
bacteremia, hemoculture, microorganisms