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Acta Medica Medianae
Vol. 45
Number 1, Januar, 2006
UDK 61
YU ISSN 0365-4478

 

Contact:
Radmila Gudovic
Institute of anatomy Faculty of Medicine
2
Hajduk Veljko Street
21000 Novi Sad, S
erbia and Montenegro
Tel.:021/6615751
E-mail
:d.maric@Eunet.yu

 

Copyright 2006 by Faculty  of Medicine, University of Nis

STEREOLOGICAL ANALYSIS OF HUMAN CEREBELLAR GRANULE CELLS IN DEVELOPING BRAIN

Radmila Gudovic, Dusica Maric and  Nada Mihic

Institute of anatomy  Faculty of Medicine Novi Sad

The aim of this investigation was to determine the numerical density of human cerebellar granule cells in three different parts of cerebellar cortex i.e. hemispherium, vermis and flocculus. Stereological analysis of human cerebellar granule cells was performed at various stages of fetal development (15 th to 31 st gestation week) and one brain of a six day-old newborn. The brains were fixed in formalin, embedded in paraffin and cut in frontal plane in 6,15, and 30 m. The slices were stained with Cresyl-violet. Each fifth slice was analyzed using the light microscope, and numerical density of granule cell nuclei was performed by point counting method using multipurpose test system M42.  The total of test fields was counted in each stage. The analysis of the obtained value for the numerical density of the nerve cell nuclei indicates a constant increase during development in all investigate parts of the cerebellar cortex. Acta Medica Medianae 2005;45(1):47–51.

 Key words: human, cerebellum, granule cell, development, stereology