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Acta Medica Medianae
Vol. 45
Number 3, July, 2006
UDK 61
YU ISSN 0365-4478

Mirjana Abramović

Department of chemistry of Faculty  of Medicine

81dr Zoran Djindjic Street

18000 Nis

Phone: 018/22 66 44 lok 113


Copyright 2006 by Faculty  of Medicine, University of Nis


 Mirjana Abramovic, Radmila Pavlovic and Gorana Rancic


Department of chemistry of Faculty  of Medicine of Nis1

Institute of Histology and Embriology of Medical Faculty of Nis


Our methodological procedure established successful technique of the direct dying of elastic fibers and lamellas in elastic tissues. Experiments were carried out on a human autopsy material. Blocks rich in elastic fibers were fixed by 10% buffered neutral formaline and cut in the thickness of 5mm. Deparaffinization of slides is done by xylol treatment (2x15 min). Additional denaturation is achieved by combined solution, which contains 1.2 g of picric acid in 30% solution of glacial acetic acid. By chloramine B application as blocking agent in form of 1% DMSO solution the dye's affinity for eviromental tissue is decreased. Tissues prepared in this way are treated with 0.5% solution of acidic sulfonic color Evans Blue (C.I.23860) in ratio 2:1. Lamellas are dark violet with light violet periphery. Obtained results are in favor of high applicability of this method in light microscopy. In our opinion, this method can be recommended as one of the methods for identification of elastic tissues. Acta Medica Medianae 2006;45(3):40-42.


Key words: elastic tissue, hystochemical identification