Acta Medica Medianae
Department of Obstetrics and Gynecology, Medical Center and Clinic
34000 Kragujevac, Serbia
Copyright 2007 by Faculty of Medicine, University of Nis
ORAL HORMONAL CONTRACEPTION - THE INFLUENCE ON HUMAN GENOME AND LIPID STATUS
Micronucleus test is the method which is used in discovering the chromosome aberrations in the cells which are exposed to the effects of chemical mutagens. The cell which suffered the DNA damage cytologically fits to extranuclear bodies in cytoplasm, which represent the chromosomal fragments, the whole chromosomes or the groups of chromosomes. These cytoplasmic masses look like small cores, micronucleuses, and their size varies depending on the size of chromosome frag-ments or the number of chromosomes which are in the cytoplasm.
The aim of the paper was to examine the influence of monophased, combined, low-dosed contraceptive pills which contain ethinyl- estradiol and gestoden on the micronucleus frequency in human lymphocytes of peripheral blood and their influence on the lipid status of the users.
We examined 30 patients older than 18 years. All the patients took part in the research in order to prevent unwanted pregnancy.The existence of cardiovascular, endocrinological, neurological and malignant diseases was excluded by using clinical examinations, biochemical, hematological and additional researches.
After the therapy of 20 µg of ethynil- estradiol and 75 µg of gestoden in the period of six consequtive menstrual cycles in order to prevent unwanted pregnancy, there was not statistically significant change of micronucleus frequency in lympho-cytes of peripheral blood in the cases of patients which took part in the research; (p> 0,05).
The therapy significantly increases the value of cholesterol, low- density lipo-proteins (LDL) and triglycerides in the blood of the patients (p< 0,05).
The therapy can be applied along with the regular control of the gynecologist in order to prevent unwanted pregnancy. Acta Medica Medianae 2007;46(1):11-16.
Key words: gestodene, ethinyl estradiol, micronuclei, genotoxicity, lipoproteins