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APPLICATION OF DIRECT CONTACT TEST IN EVALUATION OF CYTOTOXICITY OF ACRYLIC DENTURE BASE RESINS
Milena Kostić1, Stevo Najman2, Jelena Najdanović2, Nebojša Krunić3 and Ivan Kostić2
Clinic of Dentistry Niš, Department of Prosthodontics, Niš, Serbia1
University of Niš, Faculty of Medicine, Institute of Biology and Human Genetics, Niš, Serbia2
Clinic of Dentistry Niš, Department of Prosthodontics, Serbia, Niš3
The use of acrylic denture base resins is widely spread in dental practice. They belong to the group of biomaterials due to their role of morphological and functional substituent in the mouth. However, clinical practice has shown that some toxic ingredients of these materials may lead to adverse local and even systemic changes.
The aim of the study was to evaluate cytotoxic effect of various denture base resins on cell culture using direct contact test.
The effect of four different acrylic materials on HeLa cell structure was evaluated. Upon light microscopy analysis, MTT test was performed without previous removal of material samples.
The obtained values of MTT indicate that cell proliferation is dependant on the type of acrylic denture base resins. Cold polymerization denture base resins showed mild inhibitory effect on the cell culture growth. The signs of toxicity were not observed in heat polymerization denture base resins. Acta Medica Medianae 2012; 51(1):66-72.
Key words: acrylic denture base resins, direct contact test, HeLa cells