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Acta Medica Medianae
Vol. 52, No 1, March, 2013

UDK 61
ISSN 0365-4478(Printed version)
ISSN 1821-2794(Online)


Correspondence to:

Milena Ilić

Institute of Pathology

Faculty of Medical

Science University of Kragujevac

Svetozara Markovica 69

Kragujevac 34000, Serbia

E-mail: lena.ilic@gmail.com

Original article                                                   

UDC: 618.19-006:599.323.4]:618.11

UDC 615.277:[618.11:599.323.4






Milena Ilić1, Maja Zečević2, Nina Jančić3, Nataša Djindjić3, Ivan Rančić3, Dalibor Jovanović1 and Tomislav Jovanović4


University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia1

Clinic of Pediatric Surgery and Orthopedics, Clinical Center Niš, Serbia2

University of Niš, Faculty of Medicine, Niš, Serbia3

University of Belgrade, Institute of Physiology, School of Medicine, Belgrade, Serbia4




The aim of this study was to estimate ovarian changes in 7,12 dimethylbenz (α) anthracene (DMBA) induced rat mammary carcinomas. The study was carried out on female virgin albino Wistar rats (n=35, age=35-37days, body mass 120-140g), divided into control (n=10) and experimental group (n=25). Anesthetised animals of experimental group were inoculated with 2 mg mixture (1 mg of DMBA and 1 mg of cholesterol-buffer) into the fifth left mammary gland. The animals were sacrificed 90 days after implantation, and ovaries and mammary glands were investigated. Mammary gland carcinomas (in situ and/or invasive) were pathohistologically verified in 19 experimental animals. Histological, histochemical, and immunohistochemical (cytokeratin AE1/AE3  and  PCNA ) studies of ovaries were performed.

Besides non-neoplastic changes, such as decrease in ovary’s volume, reduction in the rate of follicular development and numerous corpora lutea formation were found in the vicinity of preneoplastic changes: papillomatous epithelial hyperplasia and inclusion cysts, microglandular formations with dysplasia and seromucinous microcystic formation. Intensive diffuse PCNA expression was present in the epithelium of glandlike structures, follicular and inclusion cysts.

These morphological changes confirmed that DMBA is a pluripotent carcinogen capable to induce a wide spectrum of preneoplastic lesions in the ovaries. The present dilemma is whether the changes described are the consequence of the direct effects of DMBA or of hormonal activity of the induced breast carcinomas, or both. Acta Medica Medianae 2013;52(1):25-32.


      Key words: rat, mammary glands, ovary, carcinoma, DMBA