ACTA FAC. MED. NAISS. 2004; 21 (1): 43-48

Original article

 

HISTOCHEMICAL CHANGES AND CHANGES OF ENZYME ACTIVITY OF SUCCINATE DEHYDROGENASE AND GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN THE LIVER OF THE RATS TREATED BY GENTAMICIN, VERAPAMIL AND ENALAPRIL

    Nenad Stojiljković1, Dragoljub Jovanović1, Slavimir Veljković1, Goran Ranković1,
    Voja Pavlović1, Dragan Radovanović2, Milan Stoiljković3

    1 Department of Physiology, Faculty of Medicine, University of Nis
    2 Faculty of Physical Education, University of Nis
    3 Faculty of Medicine, University of Nis

 

      SUMMARY

 

    Histochemical changes and changes of enzymic activity of succinate dehydrogenase and glucose-6-phospfate dehydrogenase in the liver of the rats treated by gentamicin, verapamil and enalapril were examined. In experimental group of animals (8 rats) treated by gentamicin (100 mg/kg TT/24 h), verapamil (3mg/kg/ TT/24 h) and enalapril (1mg/kg TT/24 h) during a period of eight days it was found that the reduction of glycogen was very prominent in intermediary and periportal zone of acinus (+ -), while it was significantly reduced in peri venular zone (+). The greatest activity of SDH in experimental group of rats was present in peri venular zone of acinus (++), reduced activity of this enzyme was found in intermediary zone (+), while the activity of SDH in periphery zone of acinus was not present at all (-). Activity of G-6-PDH enzyme was reduced in complete lobulus with the most prominent reduction (+ -) in periphery zone of acinus. In control group of animals (8 rats) treated by physiological solution (1ml/kg TT/24 h) normal distribution of glycogen was present in the all three zones (+++). Activity of SDH enzymes was most prominent in peri venular zone of acinus (+++), while in the intermediary and periportal zone the activity of this enzyme was preserved but, it was slightly weaker (++). Activity of G-6-PDH enzyme was prominent in peri venular and intermediary zone (+++) while it was not so prominent in periportal zone of acinus (++). The results of comparative study of histochemical and enzymohistochemical changes confirm the presence of significant correlation between the application of gentamicin, verapamil and enalapril as well as the above mentioned changes.

    Key words: gentamicin, verapamil, enalapril, glycogen, SDH, G-6-PDH