ACTA FAC. MED. NAISS. 2004; 21 (2): 85-88

       Original article 

    GLUTHATIONE CONTENT IN BLOOD AND KIDNEY IN GLYCEROL INDUCED ACUTE RENAL FAILURE

    Danica Tiodorović, Tatjana Cvetković

    Institute of Biochemistry, Faculty of Medicine, University of Nis

    SUMMARY

    Acute renal failure (ARF) induced by intramuscular injection of hypertonic glycerol solution was followed by free radical production. In these experimental conditions antioxidative profile is changed especially in kidney. Glutathione (GSH) as a very important nonprotein sulfhydryl component has a significant role in tissue protection from oxidative stress. In this study Wistar male rats treated with glycerol (8 ml/kg BW, 50% solution) are sacrificed after 48, 72 and 96 hours. The control group was injected by saline solution. GSH content was measured in blood and kidney tissue by Ellman reagent. Concentration of urea and creatinine, parameters of renal failure, were significantly increased in ARF group sacrificed after 48 and 72 hours, compared to the control (p<0.05). Ninety six hours after glycerol induced ARF, renal function was improved and these parameters reached normal values. The content of GSH in blood was not significantly changed in comparison to the control group, while in kidney it showed a significant decrease 48 hours after glycerol induced ARF (p<0.001). The obtained results point out the important role of GSH in kidney and suggest the possibility of preventing renal failure using GSH esters.

    Key words: glutathione, acute renal failure, oxidative stress