ACTA FAC. MED. NAISS. 2005; 22 (3): 135-138

Original article

CHARACTERISTICS OF GROWTH OF MICE BONE MARROW CULTURES IN PRESENCE OF BIOMATERIALS BASED ON HYDROXYAPATITE AND POLY-L-LACTIDE

Stevo Najman¹, Perica Vasiljević², Ljubiša Đorđević², Vojin Savić¹

¹Institute of Biomedical Research, Faculty of Medicine, Niš, Serbia
²Department of Biology with Ecology, Faculty of Science, Niš, Serbia

SUMMARY

Biodegradable hard polymers have wide medical application, especially as amend bone defects in the form of implants, bone cement, or matrices for tissue engineering. Examination of biocompatibility includes the evaluation of effects of physiologic environment on material and material on biologic ambience. Therefore, it is very important to assess possible cytotoxicity of biomaterials, their influence on cell morphology, growth division in direct or indirect contact with the material. The purpose of this study was to examine in vitro effects of different biomaterials on the growth and bone marrow cells of mice. Biomaterials HAp/PLLA with poly-L-lactide 50000 D(HP3) and 430000 D (HP2), titanium plates covered with HP2, and PLLA 200 000 D were examined. Bone marrow cell culture were maintened in MEM medium, 14 days on 33°C and 5% CO₂, and after that stained with Giemsa. The highest cell density was found in cell culture without biomaterial, and the lowest in HP2. The cell phenotype is much more diverse in control culture than we found in the presence of biomaterials. Adherent phenotype was seen with many phylopodic, lamelipodic and pseudopodic extensions. Also, there was a cell change as vacuolization, azurofilic granules and exocentric placed nucleus. Prominent phagocytosis of biomaterial HAp/PLLA which is comparable with the same process in nature bone demonstrates its good biocompatibility.

Key words: biomaterials, cell growth, cell culture, bone marow