ACTA FAC. MED. NAISS. 2005; 22 (3): 139-144

Original article

CHARACTERISTICS OF GROWTH OF MICE BONE MARROW CULTURES IN PRESENCE OF BIOMATERIALS BASED ON HYDROXYAPATITE AND POLY-L-LACTIDE

Slavica Golubović, Ivanka Đorđević, Milan Radović, Grozdana Pejović, Ivana Stanković
Clinic for lung diseases and tbc, Knez Selo,
Clinical Centre Ni{, Serbia and Montenegro

SUMMARY

The aim of the study was to assess the following parameters such as clinical and laboratory signs of inflammation syndrome, the level of diabetes regulation, localization of pulmonary infiltrates as well as bacteriological analysis of sputum specimens on acid-fast resistant bacilli and nonspecific bacterial and fungal flora in diabetic patients hospitalized with symptoms of infection and radiological infiltrates in pulmonary parenchyma confirmed during the investigation to be specific on the admission. All the examined parameters were compared with values of the patients treated for tuberculosis (TB) without associated diabetes mellitus (DM). In the study group (SG) there were 60 patients treated for TB and DM, and in the control group (CG) there were 60 of them with TB without concomitant diabetes mellitus. Average age in the SG was 59,5 years and in the CG 59 years. 40% of examinees were over 64 years of age. The average duration of symptoms in the SG was 9 and in the CG 8,9 weeks. Fever was detected in 66.7%of the SG patients and in 61.7% of the CG patients. Leucocytosis was in ratio of 48.3/35%. Cough and expectoration were present in 71.7% of the SG and 63.3% of the CG. 90% of examinees had poor regulation of serum glucose level. There were atypical lung infiltrates on the chest X-ray in 20%of examinees in relation to 1.7%of the CG patients (p<0.01). On smear microscopy, acid alcohol resistant bacilli (ARB) were isolated in ratio of 38.3/30.0% and on Low culture ARB was isolated in ratio of 68.3/36.7%. Nonspecific bacterial and fungal flora were isolated in sputum in ratio of 68.3/36.7% (p<0.001). The most frequent bacterial flora in the SG was E. coli in almost 25% of those in whom nonspecific flora was isolated. Identification and differential diagnosis of specific from nonspecific etiology of pulmonary infection in diabetic patients is important because of timely treatment in immunocompromised host.

Key words: tuberculosis, diabetes mellitus, infection