Originalni rad ACTA.FAC.MED.NAISS. 1999; 16(4), 217-225

COMPARATIVE PATHOHISTOLOGICAL, HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDY OF THE D-GALACTOSAMINE HEPATOTOXIC EFFECT AND INFLUENCES OF IMPULSE MAGNETIC FIELD ON LIVER REGENERATION AFTER D-GALACTOSAMINE HEPATITIS

Stanković Ivona , Tasić-Dimov Desanka, Kostić Olga, Kojović Zoran, Lazović Milica
1. Clinic of Physical Medicine, Rehabilitation and Protetics, Clinical Centre, Niš
2. Institute of Pathology, Medical Faculty, Niš

D-galactosamine (D-GAL) belongs to the group of indirect hepatotoxic agents with cytotoxic effects on the liver parenchyme cells. D-galactosamine hepatitis in rats is an experimental model of human viral hepatitis.

Magnetic therapy is nowadays widely applied in different chronic inflammatory conditions, degenerative diseases of locomotor system, injuries and painful conditions, but the precise mechanism of impulse magnetic field (IMF) remains unknown. In many experiments in vivo and in vitro conditions positive and stimulatory effect of IMF has been confirmed, while the data concerning it's effect on hepatic regeneration process are very rare.

In this study, we examined the hepatotoxic effect of D-GAL during subacute intoxication and the effect of IMF on liver regeneration process after induced hepatitis. D-GAL was administrated intraperitoneally during three consecutive days in individual dose of 400 mg/kg body weight. Experimental animals were treated with IMF using ELEC SYSTEM for 12 days (8 mT, 12 Hz, 30 min per day). It was noticed that D-GAL induced hepatic lesions that were morphologically similar to those in human viral hepatitis with multiple focal necrosis. Electron microscopy confirmed the characteristic ultrastructural changes of hepatocytes including microsegregation of nucleolus. Application of IMF reduced intensity and range of degenerative hepatocyte lesions; it activated and stimulated Kupffer cells and increased hepatic regeneration if compared with regenerated liver in unthreated animals. Electron microscopy, in both experimental groups (with or without IMF therapy) revealed cells with ultrastructural characteristics of regenerative hepatocytes. Cells with dilatated smooth and rough endoplasmatic reticulum, mitochondrial lesions and bigger number of secondary lysosomes were more rare in the animals treated with IMF. Our results show that IMF had positive and stimulative effects on liver regeneration process with restoration of normal architecture.

Key words: D-galactosamine, hepatitis, light microscopy, electron microscopy, liver regeneration, impulse magnetic field.