Acta Medica Medianae
Vol. 40
No 1, 2001
UDK 61
YU ISSN 0365-4478

 



Contact:
Vuka KATIĆ
Institute for Pathology of the Clinic Center

THE ROLE OF THE HYPOTHALAMUS LESIONS IN WOMEN'S STERILITY - AN EXPERIMENTAL STUDY

Vuka KATIĆ, Stevo NAJMAN, Telena ŽIVANOV-ČURLIS, Vladmila BOJANIĆ, Marko MATIJAŠ and Snežana JANČIĆ

Institute for Pathology of the Clinic Center, Institute for Biology with Genetics of the Faculty of Medicine and Clinic for Neurology of the Clinic Center, Niš

The anovulatory cycles and amenorrhea in the female patients with Cushing's syndrome, coupled with sterility, the Cushingoid type of obesity in our experiment as well as contradictory data about sterility of rats and mice in the identical experiment represent the reasons for studyng the function and morphology of the ovaries in the animals treated by monosodium glutamate (MSG).
The experimental group of the black mice C57BL/6.T of the female gender has been treated by an intraperitoneal solution of MSG in the dose of 4,4 mg/g of the body-weight from the first to the ninth day after birth. The coupling of the treated females with the untreated males was done after 90 days of age; these females were sacrificed 120 days after birth. The ovaries were removed and fixed in the solution of 10% fonnaldehyde, manually treated and cut on the microtone. The paraffin sections were colored by HE, PAS and Van Giesen methods. The identical procedure was also appliced to the control group of animals of both sexes.
All the treated animals were sterile. The ovaries were enlarged, cystic, without yellow or albinic bodies. In the control females, there were Graf follicles found in various phases of maturation as well as luteinized stroma.
The paper discusses the disturbance of the hypothalamus-hypophysis-gonad axis caused by the damage of the hypothalamus regions secreting various "releasing" hormones.

Key words: Hypothalamus obesity, monosodium glutamate, cystic ovaries