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Acta Medica Medianae
Vol. 41
No 6, 2002
UDK 61
YU ISSN 0365-4478

 




Contact:
Miodrag RADOVIĆ
Gynecological and Obstetric Clinic, Clinic for Onco!ogy in Nis

 

 

IMMUNOCYTOCHEMICAL EXPRESSION OF IMMUNOGLOBULIN G (IgG) IN THE EPITHELIUM TUMORS OF THE OVARIES

 

Miodrag RADOVIĆ, Zoran POP-TRAJKOVIĆ, Slaviša JANČIĆ, Zoran MILOSAVLJEVIĆ, Katarina KATIĆ and Snežana JANČIĆ

 

Gynecological and Obstetric Clinic, Clinic for Onco!ogy,

Institutefor Pathology ofthe Clinic Center, Niš

The Institute for Pathology of the Faculty of Medicine, Kragujevac

 

Regarding scarce studies about the G immunoglobulin distribution both in the normal and in the tumor tissue of the ovary, the aim of the paper is to examine immuno-exspression of the IgG in benign, "borderline" and malign ovary tumors.

The operative material that was first being fixed in 10 formaldehyde, treated in the routine way and molded in paraffin was used. The method that were applied upon the sections of 5 micrometers in thickness were the routine H&E method for pathohistological tumor diagnostis, the mucinohistochemical AB-PAS (pH 2,5) method for verification ofmucinous tumors ofthe ovary and the immunocytochemical SAB technique with anti-IgG antibodies (1:800).

No IgG-immunoreactive cells were verified in benign cells whik in the "bor-derline" lesions rare and individual tumor cells contain the IgG but their immunoreactivity is of poor intensity; sometimes the IgG was only present in slight traces. The intensive IgG expression was found in polymorphous, hyperchromium cells of adenocarcinoma; in these cases, the deposits of this marker were conspicuously prominent in malign tumors ofpoorer differentiation. Mononucleous inflammatory cells and macrophages of the stroma of both benign and malign cells also show intensive immunoreaction to the IgG.
The authors discuss anti-tumor role of the IgG in immunity and they suggest that the immunoglobulin G should also be introduced into the PAN marker family for "screening" ovary tumor analyses.

 

Key words: Epithelium ovary lumors, immunocytochemistry, tumor markers, immunoglobulin G