Acta
Medica Medianae
Vol. 43
Number 2, April, 2004
UDK 61
YU ISSN 0365-4478
Contact:
Nenad Stojiljković,
Institute of fiziology, Faculty of Medicine
81 Dr Zoran Djindjic Street,
18000
Niš, Serbia and Montenegro
Phone: 018/326-644 lok. 132,
e-mail: ne-sto@eunet.yu
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HISTOLOGICAL AND HISTOCHEMICAL CHANGES IN THE LIVER OF RATS TREATED BY
GENTAMICIN AND ENALAPRIL
Nenad Stojiljkovic,
Dragoljub Jovanovic,
Slavimir Veljkovic,
Goran Rankovic,
Voja Pavlovic,
Dragan Radovanovic and
Milan Stoiljkovic
Institute of fiziology,
Faculty of
Medicine, Nis*
Faculty of
phisical culture, Niš**
Faculty of
Medicine, Nis***
We have examined histological and histochemical changes in the liver of rats
treated by Gentamicin and Enalapril.
Using the experimental group of animals (10 rats) treated by Gentamicin in the
dosage of 100 mg/kg BW/24h and Enalapril in the dosage 1 mg/kg/BW/24h during the
period of 8 days, it was found that in all lobuluses of the liver, changes were
concentrated around periportal and intermediary zones. In the cytoplasm of these
hepatocytes small fatty drops were found, so that they suppressed the nucleus
and the borders of cytoplasm towards the cell membrane. Simultaneously, rare
acidophilic hepatocyte regeneration was present. Glycogen reduction (+) in the
experimental group of rats was proportional to the distribution of the changes
characterized by fatty micro-drops. In the intact perivenular zone of acinus,
larger quantities of glycogen were found (++).
In the control group of animals (10 rats) treated by physiological solution
(1ml/kg/BW/24) a clear lobular composition of the liver was present. All
hepatocytes were polygonal in shape, their cytoplasm was pink and the
localization of their nucleus was central or paracentral. There was no
noticeable difference in the form of perivenular, intermediary and periportal
zones. Control group hepatocytes were organized trabecularly with sinusoidal
capillaries in between, covered with smooth endothelial and Kupfer's cells,
characterized by ovoid nuclei and distinctively light nucleoplasm. Glycogen was
deposited in the entire cytoplasm in substantial amounts (+++), in the shape of
purple-red granules. Glycogen distribution was uniform in all zones.
The histological or micro-morphological changes in hepatocytes caused numerous
liver dysfunctions, which primarily included the liver's role in metabolic and
detoxication processes. The effects of drugs applied either directly or
indirectly (via metabolites created) resulted in degenerative and reactive burn
changes. The results of a comparative study of histological, histochemical and
biochemical changes point to the conclusion that there is a significant
correlation between the application of Gentamicin and Enalapril and the changes
mentioned above. Acta Medicae Medianae 2004; 43 (2): 19-22.
Key words: liver, Gentamicin, Enalapril
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