THE INFLUENCE OF CA-ASCORBATEON CHANGING THE ILEUM SENSITIVITY

Voja Pavlović
 

Institute of Physiology, Faculty of Medicine, Niš

 

    In the case of previously mentioned Hartley guinea pigs, male gender, weight from 250 to 300 g, which lived under normal lab’s conditions. The effect of different Ca-ascorbate concentrations was studied. Various Ca-ascorbate concentrations were used starting from 10-6 M to 10-1 M, while the sensitivity of termal part of ileum was determined by Shultz-Dale’s reaction.

    The samples of ileum treated by 10-5 M histamin and the received contraction has served as a maximal response (100%). Ileum sample was rinsed by Thyrode’s sollution, the media was exchanged by different contractions of Ca-ascorbate, and with the help of Shulzt-Dale’s reaction, determined by the intensity of ileum contraction.

    The received results show that, with the growth of Ca-ascorbate concentrartion, from 10-6 M till 10-3 M. There is a mild growth of vitamin C concentration. This is in order to get 50% of response, and it’s the greatest when 10-1 M Ca-ascorbate concentration is applied.

    These results show that the application of Ca-ascorbate lessns the sensitivity of termal ileum part. Acta Medica Medianae 2004; 43(4): 13–17.

 

    Key words: Ca-ascorbic, vitamin C, asscorbic acid, histamin, ileum, sensibilisation, Schultz-Dale-ova reaction