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Acta Medica Medianae
Snezana Cekic |
HistopatHologiC CHanges at tHe HypotHalamiC, adrenal and tHymiC
NUCLEUS ARCUATUS in rats treated witH monosodiUm glUtamate Snezana Cekic1, Milos Filipovic2, Voja Pavlovic1, Milan Ciric1, Milkica Nesic1, Zorica Jovic3 and Suzana Brankovic1
Institute of Physiology, Nis1
The aim of
this paper was to investigate the impact of neurotoxic monosodium
glutamate (MSG) on a rat’s hypothalamus, adrenal gland and thymus
nucleus arcuatus (ARCN). In newborn animals subcutaneous MSG
injections induce lesions at the hypothalamic nucleus arcuatus level
and induce hypothalamic-hypophyseal-adrenal-thymic axis disorders.
Experimental and control group of animals included 10 Wistar rats
each. Experimental group animals were treated with monosodium
glutamate (4 mg MSG/g BW) on the 2nd ,4th ,6th ,8th and 10th day of
their postnatal life. Five animals from experimental and control
group were sacrificed 7 days after MSG treatment for the purpose of
studying histopathological changes on the thymus. The remaining
animals were sacrificed 6 months after MSG treatment in order to
study histopathological changes in ARCN level and adrenal gland.
Paraffin sections of the hypothalamic tissue, adrenal gland and
thymus were hematoxylin-eosin (HE) stained. Macroscopically, the
treated animals demonstrate skeletal development arrest and
cushingoid type of obesity («buffalo type»). Histopathological
analysis of nucleus arcuatus in experimental animals demonstrates
significantly reduced number of neurons. Other cells express
degenerative changes in the form of pyknotic nuclei. Adrenal glands
in experimental rats demonstrate cortex hyperplasia, with clearly
enlarged reticular and fasciculate areas and signs of hemorrhagic
necrosis in the medulla. At thymus level, depletion of thymocytes in
the cortex is observed with hemorrhage in the medulla, coupled with
degeneration of tissues cytoarchitecture . These findings suggest
the impaired hypothalamic-hypophyseal adrenal-thymic (HPTT) axis
function in newborn rats treated with MSG. Acta Medica Medianae
2005; 44 (3):
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