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Acta
Medica Medianae
Vol. 45
Number 2, April, 2006
UDK 61
YU ISSN 0365-4478
Contact:
Dusan Sokolovic
Department of biochemistry
Faculty of Medicine
81 Dr Zoran Djindjic Street
18000 Nis
Serbia
E-mail:
soko@medfak.ni.ac.yu
Copyright 2006
by Faculty of Medicine, University of Nis
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EFFECT OF PUTRESCINE ON INTENSITY OF LIPID
PEROXIDATION IN RATS’ BRAIN WITH CHOLESTASIS
Dusan Sokolovic 1,
Gordana Bjelakovic 1, Jelenka Nikolic 1, Gordana
Kocic 1, Boris Djindjic 2, Dusica Pavlovic 1,
Stojan Radic 2, Jelena Basic 1, Marko Jovic
and Dimitros Koutsonanos 3
Department of
biochemistry
Faculty of Medicine of Nis1
Department of Pathofiziology Faculty of Medicine of Nis2
Hospital of Thessaloniki 3
Encephalopathy in cholestasis results from accumulation of unconjugated
bilirubin (UCB) and hydrophobic bile acids (BA). Toxic BA and UCB
induces neurotoxicity (apoptosis of neurons). Putrescine, spermidine and
spermine are endogenous polyamines essential for cellular growth,
regeneration and differen-tiation. Beneficial effects of putrescine in
CNS injury have been attributed to
anti-apoptotic ant anti-oxidant properties.
The aim of the study was to examine the effect of putrescine at the
level of
lipid
peroxidation
in
cholestatic brain injury.
Wistar rats were divided into 5 groups: I-control, II-sham operated
rats, III-treated only with putrescine, IV-bile duct ligated (BDL) rats,
V-BDL rats treated with putrescine (150mg/kg BW ip.). The animals were
sacrificed after the 9 -day treatment.
Administration of putrescine in BDL rats reduces concentration of blood
plasma UCB and BA (29.2±3.3 vs. 43.6±5.9 μmol/l and 11.4±0.8 vs.
22.8±2.6 μmol/l; p < 0.001). The lipid peroxidation
(MDA)
was increased in brain of BDL rats (4.98±0.54 vs. sham operated rats
3.99±0.32 nmol/mg prot; p < 0.001). Putrescine decreased MDA level in
brain of V group vs. IV group rats (2.25±0.42 vs. 4.98±0.54 nmol/mg p; p
< 0.001). The amplification of cerebral activity of polyamine oxidase
(PAO)
in BDL rats (1.25±0.09 vs. sham operated rats 0.81±0.09 U/mg prot; p <
0.001), resulted in high local concentrations of 3-acetamidopropanol and
H2O2 which lead to oxidative stress and cell
death. Administration of putrescine of BDL rats, decreased activity of
cerebral PAO compared with BDL rats (1.02±0.07 vs. 1.25±0.09 U/mg prot;
p < 0.001).
Administration of putrescine in BDL rats results in normalization of
cerebral oxidative stress and has protective role after the CNS injury
in cholestasis.
Acta Medica
Medianae 2006;45(2):45-51.
Key
words:
putrescine, lipid peroxidation, cholestasis, brain |
