Acta
Medica Medianae
Vol. 45
Number 2, April, 2006
UDK 61
YU ISSN 0365-4478
Contact:
Nenad Stojiljkovic
Institute of Fiziology Faculty of Medicine
81 Dr Zoran Djindjic Street
18000 Nis
Serbia
Tel.: 018/226-644 lok 132
E-mail:
ne-sto@eunet.yu
Copyright 2006
by Faculty of Medicine, University of Nis
|
MICROMORPHOLOGICALCHARACTERISTICS OF THE LIVER AND BIOCHEMICAL ANALYSES
IN THE BLOOD OF RATS TREATED BY GENTAMICIN AND VERAPAMIL
Nenad Stojiljkovic 1
and
Milan Stoiljkovic 21
Institute of Fiziology
Faculty of Medicine of Nis 1
Institute of Farmakology University Ilinois of Chicago 2
The use
of antimicrobial drugs, especially gentamicin and calcium blocker
verapamil, may cause transitional functional damage of the liver.
The aim of this study is to explore micro-morphological changes in the
liver and biochemical changes in the blood of rats treated with
gentamicin and verapamil.
The research included 20 rats divided in experimental and control group.
The experimental group (10 rats) was treated with gentamicin (100
mg/kg/BW/24h) and verapamil (3 mg/kgBW/24 h)
for 8 days. The control group (10 rats) received physiological solution
(1 ml/kgBW/24 h) at the same time. We analyzed
micro-morphological changes in the liver and biochemical parameters in
blood: transaminase, bilirubin and glucose.
In the control group, there was a normal lobular liver structure. All
hepatocytes had polygonal shape, pink cytoplasm and the location of
nucleus was central or paracentral. Biochemical blood analysis showed
normal level of transaminase (SGOT 29.5 +/- 7.4 iu/l; SGPT 31.7 +/- 6.9
iu/l), total bilirubin (3.1 +/- 0.9 mmol/l) and glucose (4.9 +/- 0.9
mmol/l). In the experimental group of animals, hepatocytes of all three
zones were equally damaged. In the cytoplasm, we found vacuolar
degeneration, reduced condensation of chromatine in nucleus and light
nucleoplasm. Hepatocytes of the periportal zone had acidofillic
degeneration, picnotic and hiperchromatic nuclei. Biochemical blood
analysis showed high level of transaminase
(SGOT 46.4 +/- 4.7
iu/l; SGPT 50.8+/-6.1 iu/l),
total
bilirubin
(12.8+/-1.7 mmol/l)
and glucose
(9.3+/-1.8 mmol/l). There is a statistically significant difference in
biochemical parameters between the two groups (p < 0,001).
The results of our experimental study suggest that there is an obvious
correlation between application of gentamicin and verapamil and these
changes.
Acta Medica
Medianae 2006;45(2):5-9.
Key
words: gentamicin, verapamil, hepatocytes |