Acta Medica Medianae
Vol. 45
Number 2, April, 2006
UDK 61
YU ISSN 0365-4478
 

Contact:
Nenad Stojiljkovic
Institute of Fiziology  Faculty of Medicine
81 Dr Zoran Djindjic Street
18000 Nis
Serbia
Tel.: 018/226-644 lok 132
E-mail: ne-sto@eunet.yu

Copyright 2006 by Faculty  of Medicine, University of Nis

MICROMORPHOLOGICALCHARACTERISTICS OF THE LIVER AND BIOCHEMICAL ANALYSES IN THE BLOOD OF RATS TREATED BY GENTAMICIN AND VERAPAMIL

Nenad Stojiljkovic ¹ and Milan Stoiljkovic ²¹

Institute of Fiziology  Faculty of Medicine of Nis ¹
Institute of Farmakology University Ilinois of Chicago ²

The use of antimicrobial drugs, especially gentamicin and calcium blocker verapamil, may cause transitional functional damage of the liver.
The aim of this study is to explore micro-morphological changes in the liver and biochemical changes in the blood of rats treated with gentamicin and verapamil.
The research included 20 rats divided in experimental and control group. The experimental group (10 rats) was treated with gentamicin (100 mg/kg/BW/24h) and verapamil (3 mg/kgBW/24 h) for 8 days. The control group (10 rats) received physiological solution (1 ml/kgBW/24 h) at the same time. We analyzed micro-morphological changes in the liver and biochemical parameters in blood: transaminase, bilirubin and glucose.
In the control group, there was a normal lobular liver structure. All hepatocytes had polygonal shape, pink cytoplasm and the location of nucleus was central or paracentral. Biochemical blood analysis showed normal level of transaminase (SGOT 29.5 +/- 7.4 iu/l; SGPT 31.7 +/- 6.9 iu/l), total bilirubin (3.1 +/- 0.9 mmol/l) and glucose (4.9 +/- 0.9 mmol/l). In the experimental group of animals, hepatocytes of all three zones were equally damaged. In the cytoplasm, we found vacuolar degeneration, reduced condensation of chromatine in nucleus and light nucleoplasm. Hepatocytes of the periportal zone had acidofillic degeneration, picnotic and hiperchromatic nuclei. Biochemical blood analysis showed high level of transaminase (SGOT 46.4 +/- 4.7 iu/l; SGPT 50.8+/-6.1 iu/l), total bilirubin (12.8+/-1.7 mmol/l) and glucose (9.3+/-1.8 mmol/l). There is a statistically significant difference in biochemical parameters between the two groups (p < 0,001). 
The results of our experimental study suggest that there is an obvious correlation between application of gentamicin and verapamil and these changes. Acta Medica Medianae 2006;45(2):5-9.

 Key words: gentamicin, verapamil, hepatocytes