Acta
Medica Medianae
Vol. 45
Number 3, July, 2006
UDK 61
YU ISSN 0365-4478
Contact:
Nenad
Stojiljković
Institute of Fiziology of Medical Faculty
81 dr Zoran Djindjic Street
18000 Nis, Serbia
Tel.: 018/226-644, lok 132
E-mail:
ne-sto@eunet.yu
Copyright 2006
by Faculty of Medicine, University of Nis
|
MICROMORPHOLOGICAL AND HISTOCHEMICAL
CHARACTERISTICS OF A RAT’S LIVER TREATED WITH GENTAMICIN
Nenad Stojiljkovic and
Milan Stoiljkovic
Institute of Fiziology of Medical Faculty 1
Institute of Farmakology Univerzity Ilinois of Chikago 2
The
liver is a unique organ of human body because of its location and
functions which enable normal homeostasis.
The effects of different hepatotoxic substances cause micromorphological,
ultrastructural and functional changes and liver disorders.
Some researches of gentamicin toxic effect showed its hepatotoxic
effect which caused a short-term functional liver disorder.
Micromorphological and histochemical changes were examined in the liver
of the rat treated with gentamicin.
The research included 20 rats, the experimental (10 rats) and control
group (10 rats). The experimental group was treated with gentamicin
during the period of 8 days, while the control group was treated with
physiological solution.
In the experimental group of animals treated with gentamicin, slight
degenerative changes were found such as vacuolization of nucleus and
granular changes, and vacuolar parenchymatousdegeneration
(“unclearbubbling”) which are
present in the periportal and intermediar zone of lobulus. There were no
pathohistological changes in hepatocytes in the perivenular zone. Acute
vein stasis such as dilatation of the central venula and capillaries
were present. Boundary (regenerative) panel which consist of
periportally localized hepatocytes is hyperactive and is manifested in
large hyperchromic nuclei. The amount of glycogen is reduced
in hepatocytes with vacuolar degeneration. In the perivenular zone,
glycogen was present in the normal amount.
In control group of animals treated with physiological solution,
a clear lobular construction of liver was present.Hepatocytes
are of polygonal shape and pink cytoplasm and centrally or paracentrally
localized nucleus. The difference in construction of perivenular,
intermediary and periportal zone was not noticed. Hepatocyteshad
trabecular arrangement and sinusoidal capillaries in between. In the
control group of animals, glycogen was stored in a larger quantity in
the entire cytoplasm, in the form of purple-
red granules.The distribution
of glycogen in the zones was
equal.
The results of our
experimental research showed a
clear correlation between the application of gentamicin and the
aforesaid changes.
Acta
Medica Medianae 2006;45(3):24-28.
Keywords:
gentamicin, hepatocytes, glycogen |