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Acta
Medica Medianae
Vol. 45
No 4, October, 2006
UDK 61
YU ISSN 0365-4478
Contact:
Natasa Miladinovic-Tasic
Health Care Institute
50dr Zoran Djindjic Street
18000 Nis, Serbia
Phone:018/226384
E-mail: doradika@eunet.yu
Copyright 2006
by Faculty of Medicine, University of Nis
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SEROINCIDENCE OF TRICHINELLA INFECTION IN THE NISAVA
DISTRICT
Milena Misic, Natasa
Miladinovic-Tasic and Suzana Tasic
Institute of
Microbiology
and Imunology
Faculty
of Medicine
of Nis
Our
country, unfortunately, belongs to the group of countries with high
prevalence of human and animal trichinellosis. The aim of this
retrospective study was to determine the incidence of seropositive
patients during the seventeen-year period of diagnosing trichinellosis in
the Nisava District, which, so far, has been known as a region with high
prevalence of this parasitic disease.
For this study, epidemiological data were collected and analyzed using
standard protocols in to the Department of Parasitology of the Public
Health Institute Nis.
Between 1988 and 2004, 238 serum samples which came from the patients with
symptoms of trichinelosis were examined for the presence of specific
antibodies for Trichinella spiralis.
Anti-Trichinella antibodies in the patients' sera were detected by
indirect immunofluorescence assay (IIF), (INEP - Zemun) and only 20 serum
samples were tested with both indirect imunnofluorescent assay and
enzyme-linked immunosorbent assay (ELISA, DIALAB, Austria). The incidence
rates of seropositive patients for trichinellosis were calculated as a
number of seropositive patients per 100.000 inhabitants, and the number of
382.461
inhabitants in the Nisava District
according to
the latest census from 2001 was used.
Statistical data processing was performed by using statistical program
NCSS 2004 and PASS 2005 (Hintze J. 2004. NCSS and PASS. Number Cruncher
Statistical Systems.
Kaysville,
Utah.
www.NCSS.COM.).
From 1988 to 2004, it was determined that 65 patients showed the presence
of specific antibodies for Trichinella spiralis. In the observed period
the determined incidence by year ranged from 0.00 in 1989, 1993, 1995,
1996, 2003, and 2004 to 7.06 in 1990. The incidence of Trichinella
seropositive patients defined by month was the highest in February (6.24)
and January (5.2). In this retrospective study there were no significant
differences determined between the number of Trichinella seropositive
patients in relation to sex and the way of treatment (hospitalization,
out-patient treatment).
Considering the obtained data, it can be concluded that the incidence of
Trichinella seropositive patients has declined for the last 17 years and
that the Nisava District still presents the region with sporadic incidence
of this parasitic infection in humans.
Acta medica
Medianae 2006;45(4):23-27.
Key
words:
trichinellosis, incidence, immunofluorescence assay, enzyme-linked
immunosorbent assay
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