Acta Medica Medianae
Vol. 49, No1, March, 2010
UDK 61
YU ISSN 0365-4478
 

Correspondence to:

Suzana Branković

Institut za fiziologiju

Medicinskog fakulteta

Bulevar dr Zorana Đinđića 81 18000 Niš, Srbija

E-mail: brankovic.suzana@yahoo.com

Original article
UDC: 613.26:615.322

HYPOTENSIVE AND CARDIOINHIBOTORY EFFECTS OF THE AQUEOUS AND ETHANOL EXTRACTS OF CELERY (APIUM GRAVEOLENS, APIACEAE) 

Suzana Branković¹, Dušanka Kitić², Mirjana Radenković¹, Slavimir Veljković¹, Milica Kostić², Bojana Miladinović² i Dragana Pavlović²

                        Institute of Physiology, Faculty of Medicine in Niš¹

                        Integrated Academic Studies of Pharmacy, Faculty of Medicine in Niš²

In this study we present the effects of aqueous and ethanol extracts of celery (Apium graveolens L., Apiaceae) investigated on the mean blood pressure of anaesthetized rabbits and contractility of isolated atria of the rats. In our experiments were used rabbits and Wistar albino rats. The effects of extracts (0.5-15 mg/kg) on blood pressure were recorded directly from the carotid artery. Rat isolated atria was mounted in 10 ml tissue bath. An equilibrium period of 30 min was given before the application of the extracts (0.02-0.75 mg/ml). In anesthetized rabbit, intravenous administration of aqueous extracts induced least hypotensive effects (14.35±2.94%), while the ethanol extract caused the greatest fall in the blood pressure (45.79±10.86%). Hypotensive effects of the extracts were partially blocked by atropine (0.3 mg/kg), an unselective muscarinic receptor antagonist. In isolated rat atria both aqueous and ethanolic extracts of celery, exhibit a negative chronotropic and an inotropic action. Aqueous extract decreased rate of contractions for 12.88±2.74% and amplitude for 8.73±0.89%. Ethanol extract inhibited rate of the atria contractions for 34.26±5.69%, and amplitude for 25.40±3.61%. Pretreatment of the atria with atropine (1μM) partially blocked inhibitory response of aqueous and ethanol extracts. Ethanol extract of celery exhibited significantly greater hypotensive and cardio-depressant activities then aqueous extract (p<0.05). These data suggest that the aqueous and ethanol extracts of celery caused the hypotensive, negative inotropic and chronotropic effects, which could partially be mediated possibly via stimulation of muscarinic receptors. Inhibitory effect of ethanol extract was significant comparing to aqueous extract of celery. Acta Medica Medianae 2010;49(1):13-16.

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Key words: celery, extract, blood pressure, isolated atria