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Acta Medica Medianae
Vol. 50, No 3, September, 2011

UDK 61
ISSN 0365-4478(Printed version)
ISSN 1821-2794(Online)


Correspondence to:

 Ivana Binić

Clinic of Dermatology and Venerology

Clinical Center Niš

Bulevar dr Zorana Đinđića 48

18000 Niš, Serbia

E-mail: ivana.binic@medfak.ni.ac.rs

Short communication                                                     

UDC: 616.14-007.64:615.038




Antimicrobiological effects of new natural antiseptic formulation on non-infected venous leg ulcer: pilot study


Ivana Binić1, Aleksandar Janković1, Milan Miladinović2, Đorđe Gocev3, Dimitrije Janković4

and Zoran Vrućinić5



Clinic of  Dermatology and Venerology, Clinical Center Niš, Serbia1

University of Priština, Faculty of Medicine in Priština, Serbia2

Clinic of Dermatovenerology, Clinical Center Skopje, Republic Of Macedonia3

University of Belgrade, Faculty of Medicine, Belgrade, Serbia4

Clinical Center Banja Luka, Bosnia and Herzegovina5



Venous leg ulcers represent a significant public health problem that will increase as the population ages. Numerous herbs and their extracts are potentially conducive to wound healing, including the ability to serve as antimicrobial, antifungal, astringent etc. The aim of the study was to establish the in-vivo antimicrobial effects of herbal hydrogel formulation DermaplantG. The major components of the DermaplantG were the extracts of Allii bulbus, Hyperici herba and extract of Calendulae flos. A total of 12 patients with non-infected venous leg ulcers were treated twice daily, for 5 weeks, with new hydrogel formulation. All ulcers showed clinical signs of contamination or colonization without signs of systemic infection. Premoistening the swab with sterile saline was considered when the surface of the wound was dry. The tip of the swab was rolled on its side in a zigzag pattern for at least one full rotation. Standard methods for isolation and identification of aerobic and anaerobic bacteria were used.

      On baseline assessment, a large number of different types of bacteria were detected in all venous leg ulcers. S. aureus and P. aeruginosa were isolated from almost all controls.

On baseline, mixed bacterial flora (50%) was isolated in six venous leg ulcers (five ulcers with S. aureus-P. aeruginosa and one ulcer with E.coli-Enterobacter spp-P.aeruginosa). At the end of the treatment in DermaplantG group in 8 venous ulcers were detected S. aureus (66.66%) and P. aeruginosa (16.66%), and one venous leg ulcers was detected as sterile (8.33%). The number of different types of isolated bacterial species decreased significantly (P<0.05) after the use of DermaplantG herbal preparations. Therapy in DermaplantG group was administered without any side effects.

The preliminary results of this pilot study demonstrate potential antimicrobial effects of herbal therapy on non-infected venous leg ulcers. Acta Medica Medianae 2011; 50(3):40-44.


      Key words: venous leg ulcer, microbiological flora, DermaplantG hydrogel