Acta Medica Medianae
Vol. 51, No 1, March, 2012
UDK 61
ISSN 0365-4478(Printed version)
ISSN 1821-2794(Online)
 

Correspondence to:

Milena Kostić

Clinic of Dentistry Niš

Department of Prosthodontics

Blvd dr Zoran Đinđić 52

18000 Niš, Serbia

E-mail: kosticmilena@sbb.rs

Original article                                                       

UDC: 616.314:615.461: 678.744.33

doi:10.5633/amm.2012.0112

APPLICATION OF DIRECT CONTACT TEST IN EVALUATION OF CYTOTOXICITY OF ACRYLIC DENTURE BASE RESINS

Milena Kostić¹, Stevo Najman², Jelena Najdanović², Nebojša Krunić³ and Ivan Kostić²

Clinic of Dentistry Niš, Department of Prosthodontics, Niš, Serbia¹

University of  Niš, Faculty of Medicine, Institute of  Biology and Human Genetics, Niš, Serbia²

Clinic of Dentistry Niš, Department of Prosthodontics, Serbia, Niš³

The use of acrylic denture base resins is widely spread in dental practice. They belong to the group of biomaterials due to their role of morphological and functional substituent in the mouth. However, clinical practice has shown that some toxic ingredients of these materials may lead to adverse local and even systemic changes.

The aim of the study was to evaluate cytotoxic effect of various denture base resins on cell culture using direct contact test.

The effect of four different acrylic materials on HeLa cell structure was evaluated. Upon light microscopy analysis, MTT test was performed without previous removal of material samples.

The obtained values of MTT indicate that cell proliferation is dependant on the type of acrylic denture base resins. Cold polymerization denture base resins showed mild inhibitory effect on the cell culture growth. The signs of toxicity were not observed in heat polymerization denture base resins. Acta Medica Medianae 2012; 51(1):66-72.

            Key words: acrylic denture base resins, direct contact test, HeLa cells