Acta Medica Medianae
Vol. 52, No 2, June, 2013
UDK 61
ISSN 0365-4478(Printed version)
ISSN 1821-2794(Online)
 

Correspondence to:

Andrija Šmelcerović

Department of Chemistry

Faculty of Medicine University of Niš

Bulevar Dr Zorana Đinđića 81

18000 Niš, Serbia

E-mail: a.smelcerovic@yahoo.com

Original article                                                                       

UDC: 547.972.3:633.846

doi:10.5633/amm.2013.0201

KINETICS OF QUERCETIN NITRATION BY HORSERADISH PEROXIDASE

Andrija Šmelcerović¹, Nataša Sokolov¹, Živomir Petronijević²

University of Niš, Faculty of Medicine, Niš, Serbia¹

University of Niš, Faculty of Technology, Leskovac, Serbia²

In this study we investigated the kinetics of the nitration of quercetin by horseradish peroxidase. Quercetin nitration reaction was followed by recording the spectral changes over the time at 380 nm. The reaction rate increases with increasing of the quercetin concentration and follows the Michaelis-Menten type kinetics. Kinetic parameters of the studied enzymatic reaction were determined. Acta Medica Medianae 2013;52(2):5-9.

      Key words: quercetin, nitration, horseradish peroxidase