Ljiljana Kostadinović, Mirjana Apostolović, Dušan Šurdilović, Marija Igić, Olivera Tričković-Janjić

FACULTY OF MEDICINE, CLINIC OF STOMATOLOGY, DEPARTMENT OF PREVENTIVE AND CHILDREN’S STOMATOLOGY
niš, serbia, serbia and Montenegro

In our clinical practice we have very often observed gums affected by catarrhal-initial or chronic-developed gingivitis that may lead, alongside with the disease progression, to periodontitis. The immunocytochemical changes of the inflamed gingival reverberate in the anatomical and morphological gingival characteristics. With the aim to make an adequate diagnosis and thus administer suitable treatment, immunocytochemical research of the affected gums was conducted in order to determine whether the pathohistologic findings coincide with the clinical findings. The aim of the research was to determine the condition of the changed gingiva by the clinical indexes (PMA-index according to the method of Schour and Massler), to determine the nature of gingival inflammatory infiltrate, to identify the immuno-phenotypical profile of cellular by means of monoclonal antibodies of the affected gingiva, and to subtypify T and B lymphocytes in the affected gingiva. For pathohistologic and immunocytochemical analysis they took gingival samples for biopsy from 52 subjects, from the children with clear or expressed inflammation of the gingival where tooth extraction was indicated. The control group consisted of the gingival samples taken from 10 subjects who belonged to the total sample of 86 subjects and who had the healthy gingiva. The achieved results confirmed that the clinical gingival status corresponds to the pathohistologic and immunocytochemical findings, which was the main goal of the research.

Key words: gingivitis, parodontitis, pathohistologic analysis, immunocytochemical analysis