Milena Kostić¹,
Nebojša Krunić^1,2,
Stevo Najman^2,3,
Jelena Kocić^2,3,
Milena Veselinović^2,3

¹clinic of stomatology, dePartment of Prosthodontics, nis, serbia;
²medical faculty, nis, serbia;
³institut of biomedical research, nis, serbia

Acrylic resins are materials often used in dental practice, especially in denture base manufacturing. In some cases, a reaction of the oral tissues appears on the contact spots with the base of acrylic dentures. Testing materials considered of four different acrylic resins. The influence of differently concentrated acrylic extracts on the viability of HeLa cells was examined, together with the reversibility of the changes which appeared on cells’ culture. A culture that grew in an extract free medium was used as control. The estimation of HeLa cells’ viability was done by the MTT test. As the concentration of examined acrylic extracts grows, the viability of HeLa cells considerably declines, and their recovery is slower. Complete recovery of HeLa cells has not occurred in any concentration of all examined.

Key words: acrylic resins, denture base, HeLa cell culture, MTT test